|Tested species reactivity||Chicken, Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||This antibody was produced against a chemically synthesized phosphopeptide derived from the region of Pyk2 that contains tyrosine 402. The antibody was affinity purified by sequential epitope-specific chromatography, then conjugated to Alexa Fluor® 488 under optimal conditions.|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 0.2mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Phosphoproteomic Analysis Identifies Focal Adhesion Kinase 2 (FAK2) as a Potential Therapeutic Target for Tamoxifen Resistance in Breast Cancer.
44-618A1 was used in immunocytochemistry to investigate mechanisms of tamoxifen resistance
|Wu X,Zahari MS,Renuse S,Nirujogi RS,Kim MS,Manda SS,Stearns V,Gabrielson E,Sukumar S,Pandey A||Molecular and cellular proteomics : MCP (14:2887)||2015|