|Tested species reactivity||Human|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Pyk2 that contains tyrosine 881.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Human||Not Cited||The role of proline-rich protein tyrosine kinase 2 in differentiation-dependent signaling in human epidermal keratinocytes.||Schindler EM,Baumgartner M,Gribben EM,Li L,Efimova T||The Journal of investigative dermatology (127:1094)||2007|
|Mouse||Not Cited||Role of PYK2 in the development of obesity and insulin resistance.||Yu Y,Ross SA,Halseth AE,Hollenbach PW,Hill RJ,Gulve EA,Bond BR||Biochemical and biophysical research communications (334:1085)||2005|
|Rat||Not Cited||Implication of phospholipase D2 in oxidant-induced phosphoinositide 3-kinase signaling via Pyk2 activation in PC12 cells.||Banno Y,Ohguchi K,Matsumoto N,Koda M,Ueda M,Hara A,Dikic I,Nozawa Y||The Journal of biological chemistry (280:16319)||2005|
|Heat shock protein 60 inhibits Th1-mediated hepatitis model via innate regulation of Th1/Th2 transcription factors and cytokines.||Zanin-Zhorov A,Bruck R,Tal G,Oren S,Aeed H,Hershkoviz R,Cohen IR,Lider O||Journal of immunology (Baltimore, Md. : 1950) (174:3227)||2005|
|Rat||Not Cited||Glucose activates protein kinase C-zeta /lambda through proline-rich tyrosine kinase-2, extracellular signal-regulated kinase, and phospholipase D: a novel mechanism for activating glucose transporter translocation.||Bandyopadhyay G,Sajan MP,Kanoh Y,Standaert ML,Quon MJ,Reed BC,Dikic I,Farese RV||The Journal of biological chemistry (276:35537)||2001|
|Rat||Not Cited||Salicylate Inhibits Phosphorylation of the Nonreceptor Tyrosine Kinases, Proline-Rich Tyrosine Kinase 2 and c-Src.||Wang Z,Brecher P||Hypertension (Dallas, Tex. : 1979) (37:148)||2001|
||Implication of phospholipase D2 in oxidant-induced phosphoinositide 3-kinase signaling via Pyk2 activation in PC12 cells.||Banno Y,Ohguchi K,Matsumoto N,Koda M,Ueda M,Hara A,Dikic I,Nozawa Y||The Journal of biological chemistry (280:16319)||2005|