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Western blot analysis of Phospho-FMRP pSer499 in rat hippocampal lysate using a Phospho-FMRP pSer499 polyclonal antibody (Product # PA5-35389). Results show a band at ~71kDa. The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that the lysate was incubated in lambda-Ptase (400 units/100ul lysate for 30 min) before being exposed to the FMRP Ser499 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser499 of rat FMR1 conjugated to KLH|
|Storage buffer||0.1M HEPES, pH 7.5, with 0.1mg/ml BSA, 0.15M NaCl, 50% glycerol|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, chicken, human, mouse, non-human primate, sheep, Xenopus and zebrafish based on 100% sequence homology.
This antibody contains enough material to conduct 10 mini-Western blots.
Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
FMRP, FRAXA, POF, POF1, fragile X mental retardation 1 protein; fragile X mental retardation protein 1; fragile X mental retardation protein 1 homolog; fragile X mental retardation syndrome 1 homolog; fragile X mental retardation-1 protein; protein FMR-1; ragile X mental retardation protein