Invitrogen

Phospho-FOXO1/FOXO3/FOXO4 (Thr24, Thr32) Polyclonal Antibody

View all (9) FOXO1/FOXO3/FOXO4 antibodies
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

PA5-36737

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide derived from human FoxO1 around the phosphorylation site of Threonine 24
View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2553692

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 70-82 kDa.

Purity is >95% by SDS-PAGE.

Target Information

FOXO1 + FOX3 + FOX4 belong to the forkhead family of transcription factors. FOXO1 (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined.FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.FOX4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AF6q21 protein; Fork head domain transcription factor AFX1; forkhead box O3A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead box protein O3; Forkhead box protein O4; forkhead homolog (rhabdomyosarcoma) like 1; Forkhead in rhabdomyosarcoma; Forkhead in rhabdomyosarcoma-like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1; MGC120490; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7

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Gene Aliases: AF6q21; AFX; AFX1; FKH1; FKHR; FKHRL1; FKHRL1P2; FOXO1; FOXO1A; FOXO2; FOXO3; FOXO3A; FOXO4; MLLT7

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UniProt ID: (Human) Q12778, (Human) O43524, (Human) P98177

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Entrez Gene ID: (Human) 2308, (Human) 2309, (Human) 4303

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Function(s)
negative regulation of transcription from RNA polymerase II promoter ovulation from ovarian follicle initiation of primordial ovarian follicle growth antral ovarian follicle growth oocyte maturation regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter regulation of translation DNA damage response, signal transduction by p53 class mediator tumor necrosis factor-mediated signaling pathway cellular response to oxidative stress glucose homeostasis positive regulation of apoptotic process positive regulation of neuron apoptotic process positive regulation of erythrocyte differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of canonical Wnt signaling pathway neuronal stem cell population maintenance extrinsic apoptotic signaling pathway in absence of ligand regulation of neural precursor cell proliferation regulation of reactive oxygen species metabolic process blood vessel development temperature homeostasis cellular glucose homeostasis transcription, DNA-templated protein acetylation autophagy apoptotic process cellular response to DNA damage stimulus insulin receptor signaling pathway cellular response to starvation positive regulation of autophagy endocrine pancreas development cellular response to insulin stimulus negative regulation of stress-activated MAPK cascade regulation of gluconeogenesis by regulation of transcription from RNA polymerase II promoter negative regulation of apoptotic process fat cell differentiation negative regulation of fat cell differentiation positive regulation of gluconeogenesis positive regulation of protein catabolic process negative regulation of transcription, DNA-templated enamel mineralization cellular response to hydrogen peroxide cellular response to cold cellular response to hyperoxia cellular response to dexamethasone stimulus cellular response to nitric oxide response to fluoride regulation of energy homeostasis regulation of transcription, DNA-templated cell cycle arrest mitotic G2 DNA damage checkpoint muscle organ development negative regulation of cell proliferation negative regulation of angiogenesis stem cell differentiation negative regulation of smooth muscle cell differentiation negative regulation of G0 to G1 transition positive regulation of cell cycle arrest
Process(es)
RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, transcription factor binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription coactivator binding chromatin binding protein binding beta-catenin binding ubiquitin protein ligase binding sequence-specific DNA binding protein phosphatase 2A binding core promoter binding transcription cofactor binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding protein kinase binding chromatin DNA binding transcription factor binding enzyme binding identical protein binding
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