|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic phosphopeptide derived from human FKHR around the phosphorylation site of Ser256 (A-A-SP-M-D)|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 50% glycerol, 150mM NaCl|
|Contains||0.02% sodium azide|
FOXO1 + FOX3 + FOX4 belong to the forkhead family of transcription factors.FOXO1 (FKHR or ForkHead in Rhabdomyosarcoma) is a 70 kDa protein. Recent studies have shown that this protein can act as either a coactivator or a corepressor of nuclear receptor activity. This action is mediated through the LXXLL motif found in the C terminus of the FOXO1A protein. The specific function of the gene has not yet been determined.FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.FOX4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AFX; AFX1; FKHR; FKHRL1; forkhead box O1a; forkhead box O1A (rhabdomyosarcoma); Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; forkhead protein 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma; FOXO1; FOXO1A; FOXO3; FOXO3A; FOXO4; MLLT7
Gene Aliases: Afxh; AI876417; FKH1; FKHR; Fkhr1; FOXO1; FOXO1A
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