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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of serine 253(A-V-S(p)-M-D) derived from Human FKHRL1.|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for Western blot is NIH-3T3 cells; suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is Hela cells.
FOXO3 is a ubiquitously expressed 75 kDa protein member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. FOXO3 is involved with mTOR in the regulation of autophagy in skeletal muscle, and activates protein degradation in atrophying muscle cells. FOXO3 has also been implicated in several neurodegenerative disorders including aging, neuromuscular disease, systemic lupus erythmatosus, stroke and diabetic complications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
AFX; AFX1; Drosophila); forkhead box O3A; forkhead box O4; forkhead box protein O3; forkhead homolog (rhabdomyosarcoma) like 1; forkhead in rhabdomyosarcoma-like 1; forkhead protein FKHR2; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1; FOXO4; MGC120490; MLLT7; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog
1110048B16Rik; 2010203A17Rik; AF6q21; C76856; Fkhr2; FKHRL1; FKHRL1P2; FOXO2; FOXO3; FOXO3A