|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of serine 232 (S-S-S(p)-P-A) derived from Human G3BP-1.|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
A suggested positive control for Western blot is 293 cells; suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is Hela cells.
May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium- dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ATP-dependent DNA helicase VIII; DNA helicase VIII; G3BP; G3BP-1; G3BP1; GAP binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; hDH VIII; Ras GTPase-activating protein-binding protein 1; Ras-GTPase-activating protein SH3-domain-binding protein; RasGAP-associated endoribonuclease G3BP
Gene Aliases: G3BP; G3BP1; HDH-VIII
UniProt ID: (Human) Q13283
Entrez Gene ID: (Human) 10146
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