Western blot analysis of Phospho-GABRB3 pSer408/409 in rat hippocampal lysate using a Phospho-GABRB3 pSer408/409 polyclonal antibody (Product # PA5-35390). Results show a band at ~53kDa. Immunolabeling is blocked by the phospho-peptide (lane 2) used as antigen but not by the corresponding dephosphopeptide (not shown).
|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser408/409 of rat GABRB3 conjugated to KLH|
|Storage buffer||0.1M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 0.1mg/ml BSA|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, chicken, human, mouse, non-human primate, Xenopus and zebrafish based on 100% sequence homology.
This antibody contains enough material to conduct 10 mini-Western blots.
Gamma-amino butyric acid (GAMA) is a strong inhibitor of neuron transmission and is found throughout the central nervous system. GABA is formed by the decarboxylation of glutamate, resulting in the formation of one of two distinct types; GABA-A or GABA-B.The binding of GABA to GABA-A receptors results in the increase transportation of chlorine ions into the neuron. This influx results in signal transmission repression. Studies have shown that if cell to cell signaling goes unchecked due to a lack of GABA, a system overload can resulting in blackouts, rapid body contractions, seizures and epilepsy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.