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Western blot of 10 µg of rat brain lysate showing specific immunolabeling of the ~50 kDa Gap-43 protein phosphorylated at Ser41 (Lane 1). The labeling by the antibody was blocked by the phosphopeptide representing Ser41 (Lane 2). The corresponding dephosphopeptide did not block the immunolabeling (not shown).
|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser41 of rat GAP43 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20°C or -80°C if preferred|
|Tested Applications||Dilution *|
|Dot blot (DB)||1:1000|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, chicken, finch, human, mouse, non-human primate, Xenopus and zebrafish based on 100% sequence homology.
This antibody is specific for the ~50 kDa Gap-43 protein phosphorylated at Ser41 in Western blots of rat brain lysate. In some tissues the antibody also recognizes a higher molecular weight protein, that is also recognized by the Pan Gap-43 antibody, that may be a Gap-43 aggregate or oligomer. Immunolabeling blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Gap-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenber, 1997). There is also evidence from knockout animals that Gap-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al, 1995) Gap-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by Protein Kinase C (Catalog No 1609-PKC) modulates the interaction of Gap-43 with actin (He et al, 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al, 2002).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
axonal membrane protein GAP-43; B-50; brain abundant, membrane attached signal protein 2; F1; growth accentuating protein 43; Growth Associated Protein 43; growth-associated protein 43; Nerve Growth Related Peptide; neuromodulin; protein F1