|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (Human) derived from a region of GATA 4 that contains serine 105.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.3, with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-80° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-22003 detects Phospho-GATA4 (pSer105) in Mouse samples. Expected to cross react with chicken, human and rat due to sequence homology (100% identity with immunogen).
PA1-22003 has been successfully used in Western Blot procedures, where a band of 46 kDa is predicted to be detected. Positive control: Phenylephrine-stimulated rat cardiomyocytes, transfected with mouse GATA4
PA1-22003 immunogen corresponds to Synthetic peptide (Human) derived from a region of GATA 4 that contains serine 105.
GATA 4 is a 46 kDa member of the GATA family of zinc finger-containing transcription factors that is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress-induced gene expression in cardiac and smooth muscle cell types. GATA 4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA 4 is phosphorylated in response to agonist stimulation through MEK 1-ERK 1 / 2, and weakly through JNK or p38 MAPKs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.