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Invitrogen
Phospho-GCN2 (Thr899) Recombinant Rabbit Monoclonal Antibody (PSH10-57)
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FIGURE: 1 / 13
Phospho-GCN2 (Thr899) Antibody (MA562894) in ICC/IF
Immunofluorescence analysis of Phospho-GCN2 (T899) using Neuro-2a cells untreated or treated with 100 nM Calyculin A for 30 minutes. The cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. The cells were co-labeled with Ph... View More
Product Details
MA562894
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH10-57
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Thr899 of Human GCN2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Positive Control: HeLa cell lysate, HeLa starved for 3 hours then treated with 100nM Calyculin A for 30 minutes cell lysate, Neuro-2a cell lysate, Neuro-2a treated with 100nM Calyculin A for 30 minutes cell lysate, C6 cell lysate, C6 treated with 100ng/mL Calyculin A for 1 hour cell lysate, HeLa cells treated with 100nM Calyculin A for 30 minutes, Neuro-2a cells treated with 100nM Calyculin A for 30 minutes, C6 cells treated with 100 ng/mL Calyculin A for 1 hour, human brain tissue, mouse brain tissue, rat brain tissue.
Tissue Specificity: Low tissue specificity.
Subcellular Location: Cytoplasm.
Sequence Similarities: 100% Mouse/Rat.
Predicted band size: 187 kDa.
Target Information
EIF2AK4 (GCN2) is a metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor-2 in response to low amino acid availability. It plays a role as an activator of the integrated stress response required for adapatation to amino acid starvation. Mutations in the gene lead to pulmonary vennocclusive disease 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: eIF-2-alpha kinase GCN2; Eukaryotic translation initiation factor 2-alpha kinase 4; GCN2 eIF2alpha kinase; GCN2-like protein; general control nonderepressible 2; mGCN2; unnamed protein product
Gene Aliases: 2610011M03; EIF2AK4; GCN2; KIAA1338; MGCN2; PVOD2
UniProt ID: (Human) Q9P2K8, (Mouse) Q9QZ05
Entrez Gene ID: (Human) 440275, (Mouse) 27103, (Rat) 114859
tRNA binding
nucleotide binding
RNA binding
protein kinase activity
protein serine/threonine kinase activity
eukaryotic translation initiation factor 2alpha kinase activity
ATP binding
kinase activity
transferase activity
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
eukaryotic initiation factor eIF2 binding
aminoacyl-tRNA synthetase
non-receptor serine/threonine protein kinase
translational protein
DNA damage checkpoint
positive regulation of defense response to virus by host
adaptive immune response
T cell activation involved in immune response
immune system process
positive regulation of adaptive immune response
translational initiation
regulation of translation
regulation of translational initiation
protein phosphorylation
nervous system development
learning
long-term memory
regulation of translational initiation by eIF2 alpha phosphorylation
viral translation
negative regulation of translational initiation in response to stress
negative regulation of CREB transcription factor activity
cellular response to amino acid starvation
cellular response to UV
response to endoplasmic reticulum stress
negative regulation by host of viral genome replication
negative regulation of neuron differentiation
negative regulation of translational initiation
protein autophosphorylation
defense response to virus
regulation of cell cycle
regulation of feeding behavior
cellular response to cold
positive regulation of translational initiation in response to starvation
GCN2-mediated signaling
positive regulation of long-term synaptic potentiation
neuron projection extension
cellular response to starvation
positive regulation of gene expression
phosphorylation
negative regulation of translation
endoplasmic reticulum unfolded protein response
regulation of translational initiation in response to stress
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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