Western blot analysis of Phospho-GRB10 pTyr67 using Phospho-GRB10 pTyr67 polyclonal antibody (Product # PA5-36808) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with insulin (100nM, 5min), Lane 2: Mouse spleen tissue lysate, Lane 3: PC12 cell lysate treated with insulin (100nM, 5min).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human GRB10 around the phosphorylation site of Tyrosine 67|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 67 kDa.
Purity is >95% by SDS-PAGE.
GRB7, a SH2 domain protein, has a single SH2 domain at its C-terminal, a central region with similarity to Ras GAP, and a proline-rich N terminus. A related SH2 domain-containing protein, GRB10, exhibits a high degree of homology with GRB7. GRB10 undergoes serine but not tyrosine phosphorylation in response to EGF treatment, but appears to bind to the EGF receptor poorly. GRB10 maps to mouse chromosome 11, in close proximity to the EGF receptor. Similarly, GRB7 maps to the same mouse chromosome near the EGF receptor-related protein HER2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.