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Western blot analysis of Phospho-GSK3 alpha pTyr279+GSK3 beta pTyr216 in HeLa whole cell lysates with a blocking peptide (lane 1) and no blocking peptide (lane 2) using a Phospho-GSK3 alpha pTyr279+GSK3 beta pTyr216 polyclonal antibody (Product # PA5-35482).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthesized peptide derived from human GSK3 alpha/ beta around the phosphorylation site of Tyrosine 279/216|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
FA; factor A; Glycogen synthase kinase-3 alpha; Glycogen synthase kinase-3 beta; GSK-3 alpha; GSK-3 beta; GSK3A; GSK3B; GSK3beta isoform; serine/threonine-protein kinase GSK3A; serine/threonine-protein kinase GSK3B
2700086H06Rik; 7330414F15Rik; 8430431H08Rik; C86142; GSK-3; GSK-3beta; GSK3
Carbohydrate metabolism Other polysaccharide metabolism Glycogen metabolism Protein metabolism and modification Protein modification Protein phosphorylation Signal transduction Cell surface receptor mediated signal transduction Other receptor mediated signaling pathway Intracellular signaling cascade Developmental processes Embryogenesis Segment specification Ectoderm development Neurogenesis Mesoderm development Cell cycle Mitosis Cell proliferation and differentiation