OPA1-03083 detects the alpha and beta isoforms of phospho-GSK3 from human and mouse tissues. This antibody is expected to cross-react with rat, frog, and zebra fish GSK-3 due to 100% sequence homology.
OPA1-03083 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~51 kDa protein and an ~47 kDa protein from stimulated 3T3-L1 cells representing tyrosine 279 phosphorylated GSK3alpha and tyrosine 216 phosphorylated GSK3beta, respectively. Positive control: serum starved mouse 3T3-L1 cells +/- insulin (100 nM, 10 minutes), or background extracts +/- human recombinant GSK-3â.
OPA1-03083 immunizing phosphopeptide was derived from a regions of human GSK3alpha/beta that contain tyrosine 279/216.
Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3's substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Glycogen synthase kinase-3 alpha; Glycogen synthase kinase-3 beta; GSK-3 alpha; GSK-3 beta; gsk-3-alpha; GSK3beta isoform; Serine/threonine-protein kinase GSK3A; Serine/threonine-protein kinase GSK3B
Gene Aliases: 2700086H06Rik; 7330414F15Rik; 8430431H08Rik; C86142; GSK-3; GSK-3beta; GSK3; GSK3A; GSK3B