Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Molecular Testing
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Who We Serve
    • Biotech
    • Biopharma
    • CDMO
    • Lab Diagnostics
    • Industrial and Applied Sciences
  • Promotions
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search

Search All
Search button
          • Order Status
          • Quick Order
          • Promos
          • Sign in
            Sign in
            Don't have an account ? Create Account
            • Account
            • Check Order Status
            • Aspire Member Program
            • Connect: Lab, Data, Apps
            • Custom Products & Projects
            • Services Central
          • Primary Antibodies ›
          • GSK3 alpha/beta Antibodies

          Invitrogen

          Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Polyclonal Antibody

          View all (24) GSK3 alpha/beta antibodies

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Datasheet
          Certificates
          Protocols
          Questions & Answers
          Datasheet
          Certificates
          Protocols
          Questions & Answers

          Cite Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Polyclonal Antibody

          Additional Information:
          {{banner.description}}
          {{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
          • Antibody Testing Data (1)
          Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Antibody (PA5-85792) in WB

          Western Blot using Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Polyclonal Antibody (Product # PA5-85792). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 alpha/beta antibody Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Polyclonal Antibody (Product # PA5-85792) diluted at 1:1,000. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Antibody in Western Blot (WB)
          Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Polyclonal Antibody

          Product Details

          PA5-85792

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GSK3 alpha + GSK3 beta. The exact sequence is proprietary.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.91 mg/mL

          Amount

          191 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7, with 20% glycerol

          Contains

          0.025% ProClin 300

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2792928

          Product Specific Information

          Keep as concentrated solution.

          Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Rhesus Monkey (100%), Bovine (100%).

          Positive Control: 293T, A431, HeLa, HepG2.

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3's substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

          Have you cited this product in a publication?
          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: Glycogen synthase kinase-3 alpha; Glycogen synthase kinase-3 beta; GSK-3 alpha; GSK-3 beta; gsk-3-alpha; GSK3B; kinase; Serine/threonine-protein kinase GSK3A; Serine/threonine-protein kinase GSK3B; unnamed protein product

          View more View less

          Gene Aliases: GSK3A; GSK3B

          View more View less

          UniProt ID: (Human) P49840, (Human) P49841

          View more View less

          Entrez Gene ID: (Human) 2931, (Human) 2932

          View more View less

          Function(s)
          nucleotide binding protein kinase activity protein serine/threonine kinase activity receptor binding protein binding ATP binding kinase activity transferase activity protein kinase A catalytic subunit binding tau protein binding tau-protein kinase activity protein serine kinase activity protease binding p53 binding beta-catenin binding protein kinase binding ubiquitin protein ligase binding dynactin binding NF-kappaB binding RNA polymerase II sequence-specific DNA binding transcription factor binding scaffold protein binding
          Process(es)
          G1/S transition of mitotic cell cycle epithelial to mesenchymal transition positive regulation of cell-matrix adhesion cytoplasmic translational initiation heart valve development glycogen metabolic process protein phosphorylation ER overload response mitochondrion organization regulation of mitotic cell cycle nervous system development circadian rhythm insulin receptor signaling pathway negative regulation of signal transduction positive regulation of autophagy positive regulation of gene expression negative regulation of gene expression negative regulation of epithelial to mesenchymal transition regulation of neuron projection development Wnt signaling pathway peptidyl-serine phosphorylation viral protein processing hippocampus development establishment of cell polarity maintenance of cell polarity cell differentiation positive regulation of cell migration negative regulation of cell migration regulation of axon extension SCF-dependent proteasomal ubiquitin-dependent protein catabolic process neuron projection development negative regulation of protein complex assembly positive regulation of protein complex assembly positive regulation of protein ubiquitination negative regulation of TOR signaling positive regulation of protein binding negative regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of microtubule-based process response to endoplasmic reticulum stress intracellular signal transduction cellular response to interleukin-3 TORC2 signaling regulation of circadian rhythm negative regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process protein kinase B signaling positive regulation of neuron apoptotic process positive regulation of cell differentiation negative regulation of osteoblast differentiation negative regulation of glycogen biosynthetic process positive regulation of cilium assembly positive regulation of translation positive regulation of protein catabolic process positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation regulation of protein export from nucleus positive regulation of protein export from nucleus rhythmic process regulation of dendrite morphogenesis regulation of axonogenesis positive regulation of protein kinase B signaling canonical Wnt signaling pathway excitatory postsynaptic potential regulation of microtubule cytoskeleton organization negative regulation of calcineurin-NFAT signaling cascade superior temporal gyrus development cellular response to retinoic acid negative regulation of canonical Wnt signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand presynaptic modulation of chemical synaptic transmission neuron projection organization membraneless organelle assembly regulation of microtubule anchoring at centrosome beta-arrestin-dependent dopamine receptor signaling pathway regulation of cellular response to heat negative regulation of protein localization to nucleus regulation of long-term synaptic potentiation positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway cellular response to oxygen-containing compound negative regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of protein localization to cilium negative regulation of TORC2 signaling negative regulation of dopaminergic neuron differentiation cellular response to beta-amyloid positive regulation of protein localization to centrosome positive regulation of establishment of protein localization negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of type B pancreatic cell development negative regulation of glycogen (starch) synthase activity negative regulation of mesenchymal stem cell differentiation regulation of systemic arterial blood pressure cardiac left ventricle morphogenesis negative regulation of UDP-glucose catabolic process cell migration lipopolysaccharide-mediated signaling pathway cellular response to insulin stimulus positive regulation of heart contraction negative regulation of glucose import negative regulation of insulin receptor signaling pathway negative regulation of cell growth involved in cardiac muscle cell development cellular response to lithium ion cellular response to glucocorticoid stimulus positive regulation of adrenergic receptor signaling pathway positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway autosome genomic imprinting positive regulation of substrate adhesion-dependent cell spreading regulation of mitophagy positive regulation of beta-amyloid formation positive regulation of protein targeting to mitochondrion
          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Ordering Plus Icon Minus Icon
          • Order Status
          • Order Help
          • Quick Order
          • Supply Center
          • eProcurement
          Support Plus Icon Minus Icon
          • Help and Support
          • Contact Us
          • Technical Support Centers
          • Documents and Certificates
          • Report a Site Issue
          Resources Plus Icon Minus Icon
          • Learning Centers
          • Promotions
          • Events and Webinars
          • Social Media
          About Thermo Fisher Plus Icon Minus Icon
          • About Us About Us
          • Careers Careers
          • Investors Investors
          • News News
          • Social Responsibility Social Responsibility
          • Trademarks
          • Consumer Health Data Privacy Policy Consumer Health Data Privacy Policy
          • Policies and Notices
          Our Portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Unity Lab Services
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Notice
          • Price & Freight Policy
          © 2006-2026 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          United States flag icon
          United States

          Your items have has been added!


          Host server : magellan-search-blue-7c59b9b899-97tfg:80/100.66.76.242:80.
          git-commit: 8ef7cf686e3c81d28b13492708ce56d167b2f995
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.49.0-Offline