Immunohistochemical analysis of Phospho-GSK3 alpha pTyr279+GSK3 beta pTyr216 in paraffin-embedded human breast carcinoma using Phospho-GSK3 alpha pTyr279+GSK3 beta pTyr216 polyclonal antibody (Product # PA5-36646) at a dilution of 1:100.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human GSK3 alpha around the phosphorylation site of Tyrosine 279|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 46 and 51 kDa.
Purity is >95% by SDS-PAGE.
Glycogen synthase kinase-3 alpha and beta (GSK-3 alpha, beta) are serine/threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3 beta activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNF alpha in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3 alpha and GSK-3 beta induces phosphorylation of Ser 21 and Ser 9, respectively through a phosphatidylinositol 3-kinase (PI 3-kinase)-protein kinase B (PKB) dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3 alpha or Ser 9 of GSK-3 beta and inactivates both forms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.