Western blot analysis of extracts from HeLa cells, either hydroxyurea-treated (4 mM, 20 hours) to induce G1/S phase arrest or paclitaxel-treated (100 nM/mL, 20 hours) for G2/M phase arrest, using GSK-3 (pT390) Polyclonal Antibody (top) or a GSK-3 antibody (bottom) to show induction of phospho-GSK and equal loading.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic phosphopeptide corresponding to residues surrounding Thr390 of human GSK-3-beta|
|Storage buffer||0.01M HEPES, pH 7.5, with 50% glycerol, 100µg/ml BSA, 0.15M NaCl|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Glycogen synthase kinase-3 beta (GSK3 beta) belongs to the subfamily of glycogen synthase kinases, that play a central role in energy metabolism, neuronal cell development, and body pattern formation. GSK3 has alpha and beta isoforms (51 kDa and 47 kDa respectively), that are functionally dissimilar. GSK3 beta plays a role in insulin and Wnt signaling, and is inhibited following the extracellular signaling events.
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