|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of serine 387 (R-I-S(p)-G-C) derived from Human GTPase Activating Protein.|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
A suggested positive control for Western blot is COS7 cells.
Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.
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Protein Aliases: CYK4; GTPase activating protein; GTPase activating protein ID-GAP; Male germ cell RacGap; MgcRacGAP; Protein CYK4 homolog; RAC GTPase- activating protein 1; RAC GTPase-activating protein; Rac GTPase-activating protein 1; RACGAP1
Gene Aliases: AI227039; AI327394; Band25; CYK4; gtl11; GTPase; HsCYK-4; ID-GAP; KIAA1478; MGCRACGAP; mKIAA1478; RACGAP1
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