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Western blot analysis of Phospho-Gab1 pTyr627 using Phospho-Gab1 pTyr627 polyclonal antibody (Product # PA5-36846) at a dilution of 1:500. Lane 1: MCF-7 cell lysate treated with EGF (0.1ng/ml, 30min), Lane 2: PC12 cell lysate treated with EGF (0.1ng/ml, 30min).
|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human Gab 1 around the phosphorylation site of Tyrosine 627|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 76 and 110 kDa.
Purity is >95% by SDS-PAGE.
Gab 1 (GRB2-associated binder 1), one such member of the IRS family, plays an important role in cellular growth response, transformation and apoptosis. Gab 1 is a multi-substrate docking protein that functions downstream in the signaling pathways of different receptor kinases, including EGFR. Gab1 is tyrosine phosphorylated normally in response to insulin and consequently enhances phosphatidylinositol 3- kinase (PI3K) binding. In response to osmotic shock, tyrosine-phosphorylated Gab 1 (p-Gab 1) also binds and activates phosphatidylinositol 3-kinase, suggesting that Gab 1 is the major site for PI3K recruitment following osmotic shock stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
GAB1; GRB2-associated binder 1; GRB2-associated binding protein 1; growth factor receptor bound protein 2-associated protein 1