|Tested species reactivity||Rat|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide derived from the rat GluR1 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 100µg/ml BSA, 50% glycerol, 0.15M NaCl|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct subunits GluR1-4 and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is also potentiated by phosphorylation at Ser831. In addition, phosphorylation of this site has been linked to synaptic plasticity as well and learning and memory.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Phosphorylation of the AMPA receptor GluA1 subunit regulates memory load capacity.
36-8300 was used in western blot to investigate the regulatory mechanism for memory load capacity.
|Olivito L,Saccone P,Perri V,Bachman JL,Fragapane P,Mele A,Huganir RL,De Leonibus E||Brain structure and function (221:591)||2016|
|Not Applicable||Not Cited||
Chemokine CXCL8 modulates GluR1 phosphorylation.
36-8300 was used in western blot to identify residues that affect the GluR1/CXCR2 interaction and impair CXCL8-induced cell migration
|Catalano M,Trettel F,Cipriani R,Lauro C,Sobrero F,Eusebi F,Limatola C||Journal of neuroimmunology (198:75)||2008|
AMPA receptors in the medial amygdala are critical for establishing a neuroendocrine memory in the female rat.
36-8300 was used in immunohistochemistry - free floating and western blot to assess AMPA receptor function in the medial posterodorsal amygdala
|Oberlander JG,Lin AW,Man HY,Erskine MS||The European journal of neuroscience (29:146)||2009|