View (13) other GluR1 antibodies

Invitrogen

Phospho-GluR1 (Ser845) Polyclonal Antibody

Data Sheets

Create Citation

Tech Support

Catalog # 36-8300

Check your price

Quantity:

Request bulk or custom quote

Add To Cart

What is a shared list?

Performance Guarantee

Product Image Gallery

Phospho-GluR1 (Ser845) Antibody in Western Blot (WB) — Western blot analysis of phospho-GluR1 in rat brain lysate using a phospho-Glur1 pSer845 polyclonal antibody (Product # 36-8300).

Phospho-GluR1 (Ser845) Antibody — Fig. 2 AMPA receptor phosphorylation mediates object memory capacity. a Schematic representation of the study and test phase of the object memory span task at 1-min RTI. b - e Performance of phospho-free and WT mice in the DOT-IOT task at 1-min RTI. Phospho-free mice, as well as WT animals, explored the new object ( N ) significantly more than all familiar objects ( F ) in low memory load condition (6-IOT and 3-DOT) ( b , c ). In high memory load condition (6-DOT) different from WT, they did not prefer the new object ( d ). Both WT and phospho-free mice did not prefer the new object in the 9-DOT ( e ). f Histograms report percentage increase (above the naive group) of GluA1 AMPA-R phosphorylation at S831 and S845, measured by western blot, in naive animals and animals exposed to 6 identical (6-IOT), 9 identical (9-IOT), 3 different (3-DOT), 6 different (6-DOT) or 9 different (9-DOT) objects. The results show that AMPA-R phosphorylation was significantly increased at both serine sites after the exposure to 6 different objects (6-DOT), and at S845 after the 3-DOT. * p < 0.05 new ( N ) vs. all the other familiar ( F ) objects, Duncan post hoc tests. # p < 0.05 different vs. naive group, Student's t test

Product Details

TESTED APPLICATIONS	DILUTION
ELISA (ELISA)	Assay Dependent
Immunofluorescence (IF)	Assay Dependent
Immunohistochemistry (IHC)	1:1000
Western Blot (WB)	1:1000

PUBLISHED APPLICATIONS
Western Blot (WB)	See 2 publications below
Immunohistochemistry - Free Floating (IHC (Free))	See 1 publications below

Species reactivity	Rat
Published species	Mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic phospho-peptide derived from the rat GluR1 protein.
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	0.01M HEPES, pH 7.5, with 100µg/ml BSA, 50% glycerol, 0.15M NaCl
Contains	no preservative
Storage conditions	-20°C
RRID	AB_2533280

Background/Target Information

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct subunits GluR1-4 and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is also potentiated by phosphorylation at Ser831. In addition, phosphorylation of this site has been linked to synaptic plasticity as well and learning and memory.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (3)

Have you cited this antibody in a publication? so we can reference it in this datasheet.

Bioinformatics

Protein Aliases: AMPA 1; AMPA-selective glutamate receptor 1; GluA1; GLUH1; GluR-1; GluR-A; GluR-K1; GLUR1; GLURA; glutamate receptor; Glutamate receptor 1; glutamate receptor A; Glutamate receptor ionotropic; Glutamate receptor ionotropic, AMPA 1; glutamate receptor subunit GluR1; glutamate receptor, ionotropic, AMPA1 (alpha 1); GRIA1; HBGR1; ionotropic; MGC133252

Gene Aliases: GluA1; gluR-A; Glur1; Gria1

UniProt ID: (Rat) P19490

Entrez Gene ID: (Rat) 50592

Molecular Function: ion channel ionotropic glutamate receptor ligand-gated ion channel receptor transporter

Function(s)

regulation of receptor recycling chemical synaptic transmission long-term memory response to toxic substance response to lithium ion response to organic cyclic compound neuronal action potential spinal cord development receptor internalization response to estradiol ion transmembrane transport ionotropic glutamate receptor signaling pathway synaptic transmission, glutamatergic response to cocaine response to drug response to peptide hormone positive regulation of membrane potential response to arsenic-containing substance regulation of synaptic plasticity modulation of synaptic transmission positive regulation of synaptic transmission response to electrical stimulus long term synaptic depression response to fungicide cellular response to amino acid stimulus cellular response to dsRNA cellular response to growth factor stimulus cellular response to peptide hormone stimulus cellular response to organic cyclic compound cellular response to amine stimulus

Process(es)

G-protein alpha-subunit binding
ionotropic glutamate receptor activity
AMPA glutamate receptor activity
extracellular-glutamate-gated ion channel activity
protein binding
adenylate cyclase binding
protein kinase binding
protein domain specific binding
PDZ domain binding
small GTPase binding
myosin V binding
G-protein beta-subunit binding
beta-2 adrenergic receptor binding
identical protein binding
protein homodimerization activity
protein kinase A binding

Suggested Secondary Antibodies

Anti-Rabbit Secondary Antibodies (264)

Alexa Fluor® Anti-Rabbit Secondary Antibodies (69)

HRP Anti-Rabbit Secondary Antibodies (30)

Documents

Material safety data sheets (MSDS)

Request a MSDS

Performance Guarantee

If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Phospho-GluR1 (Ser845) Polyclonal Antibody

Product Details

Background/Target Information

References (3)

Have you cited this antibody in a publication? so we can reference it in this datasheet.

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

Performance Guarantee

We’re here to help

Brands

Shopping Tool

eSolutions

Support

Thermo Fisher Scientific

Your items have has been added!

Phospho-GluR1 (Ser845) Polyclonal Antibody

×

Product Details

Background/Target Information

References (3)

Bioinformatics

Suggested Secondary Antibodies

Documents

Material safety data sheets (MSDS)

Performance Guarantee

We’re here to help

Brands

Shopping Tool

eSolutions

Support

Thermo Fisher Scientific

Your items have has been added!