Invitrogen

Phospho-GluR1 (Ser845) Polyclonal Antibody

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Phospho-GluR1 (Ser845) Antibody in Western Blot (WB) — Western blot was performed using Anti-Phospho-GluR1 (Ser845) Polyclonal Antibody(Product # 36-8300) and a 110kDa band corresponding to Phospho-GluR1 (Ser845) was observed. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Liver (Lane 3) and Rat Liver (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). For Fig. B, the blot was treated with Lambda Protein Phosphatase after the protein transfer, and for Fig. A, the blot was left untreated. In Fig.a, band of interest is not present in Mouse Liver and Rat Liver, a tissue which is reported to be negative for GluR1. Phospho-specificity is demonstrated by reduced intensity of bands in Fig.b.

Product Details

36-8300

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 5 publications 5 publications
Immunohistochemistry (IHC)	1:1,000	-
Immunohistochemistry - Free Floating (IHC (Free))	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic phospho-peptide derived from the rat GluR1 protein.
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	0.01M HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 0.15M NaCl
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2533280

Target Information

GluR1 (glutamate receptor 1) is an ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter, L-glutamate, induces a conformation change that leads to the opening of the cation channel and converts the chemical signal to an electrical impulse. Then, the receptor desensitizes rapidly and enters a transiet inactive state characterized by the presence of a bound agonist. In the presence of CACNG4, CACNG7, or CACNG8 GluR1 shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Subunit-dependent and subunit-independent rules of AMPA receptor trafficking during chemical long-term depression in hippocampal neurons.

The Journal of biological chemistry

Matsuda S,Yuzaki M

36-8300 was used in Western Blot to show that phosphomimetic mutations of the membrane-proximal region (MPR) in GluA1 AMPA-type glutamate receptors subunits affect the subunit-dependent endosomal transport of AMPARs during chemical long-term depression.

Sun Aug 01 00:00:00 EDT 2021

Caspase Activation and Caspase-Mediated Cleavage of APP Is Associated with Amyloid β-Protein-Induced Synapse Loss in Alzheimer's Disease.

Cell reports

Park G,Nhan HS,Tyan SH,Kawakatsu Y,Zhang C,Navarro M,Koo EH

36-8300 was used in Western Blotting to propose that the APP cytosolic domain plays an essential role in Aβ-induced synaptic damage in the injury pathway mediated by localized caspase activation.

Tue Jun 30 00:00:00 EDT 2020

Phosphorylation of the AMPA receptor GluA1 subunit regulates memory load capacity.

Brain structure & function

Olivito L,Saccone P,Perri V,Bachman JL,Fragapane P,Mele A,Huganir RL,De Leonibus E

36-8300 was used in western blot to investigate the regulatory mechanism for memory load capacity.

Fri Jan 01 00:00:00 EST 2016

AMPA receptors in the medial amygdala are critical for establishing a neuroendocrine memory in the female rat.

The European journal of neuroscience

Oberlander JG,Lin AW,Man HY,Erskine MS

36-8300 was used in immunohistochemistry - free floating and western blot to assess AMPA receptor function in the medial posterodorsal amygdala

Thu Jan 01 00:00:00 EST 2009

AMPA receptors in the medial amygdala are critical for establishing a neuroendocrine memory in the female rat.

The European journal of neuroscience

Oberlander JG,Lin AW,Man HY,Erskine MS

36-8300 was used in immunohistochemistry - free floating and western blot to assess AMPA receptor function in the medial posterodorsal amygdala

Thu Jan 01 00:00:00 EST 2009

Chemokine CXCL8 modulates GluR1 phosphorylation.

Journal of neuroimmunology

Catalano M,Trettel F,Cipriani R,Lauro C,Sobrero F,Eusebi F,Limatola C

36-8300 was used in western blot to identify residues that affect the GluR1/CXCR2 interaction and impair CXCL8-induced cell migration

Thu Jul 31 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: AI853806; AMPA-selective glutamate receptor 1; GluA1; GLUH 1; GluR K1; GluR-1; GluR-A; GluR-K1; GluR1; GluRK1; glutamate receptor; Glutamate receptor 1; glutamate receptor A; Glutamate receptor ionotropic, AMPA 1; glutamate receptor subunit GluR1; glutamate receptor, ionotropic, AMPA1 (alpha 1); GPRC1A; Gria 1; GRM1A; HGBR1; HIPA; MGC13325; MGC133252; NMDA1; NMDAR1; OTTHUMP00000160643; OTTHUMP00000165781; OTTHUMP00000224241; OTTHUMP00000224242; OTTHUMP00000224243; RP11-350O14.1

Gene Aliases: 2900051M01Rik; Glr-1; Glr1; GluA1; Glur-1; GluR-A; gluR-K1; Glur1; GluRA; Gria1; HIPA1

UniProt ID: (Rat) P19490, (Mouse) P23818

Entrez Gene ID: (Rat) 50592, (Mouse) 14799

Molecular Function: transmembrane signal receptor