Invitrogen

Phospho-GluR1 (Ser845) Polyclonal Antibody

2 Published Figures

4 References

View all (31) GluR1 antibodies

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Datasheet

Questions & Answers

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Phospho-GluR1 (Ser845) Antibody in Western Blot (WB) — Western blot was performed using Anti-Phospho-GluR1 (Ser845) Polyclonal Antibody(Product # 36-8300) and a 110kDa band corresponding to Phospho-GluR1 (Ser845) was observed. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Liver (Lane 3) and Rat Liver (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). For Fig. B, the blot was treated with Lambda Protein Phosphatase after the protein transfer, and for Fig. A, the blot was left untreated. In Fig.a, band of interest is not present in Mouse Liver and Rat Liver, a tissue which is reported to be negative for GluR1. Phospho-specificity is demonstrated by reduced intensity of bands in Fig.b.

Phospho-GluR1 (Ser845) Antibody — Fig. 3 In high memory load condition object LTM shares the same molecular pathway as object memory at short retention interval with the addition of novel protein synthesis. a Photos of representative Nissl-stained coronal sections of animals bilaterally implanted with permanent cannulae and injected within the hippocampus. b , c , e , f , h , i , j , k Effects of hippocampus focal pre-training and post-training administrations of inhibitors on the preference for the new object ( N ) and compared to familiar objects ( F ) in the test phase of the 6-DOT performed at 1-min and 24-h RTI, respectively. In all cases vehicle-injected animals explored the new object significantly more than all the other familiar objects. Pre-study phase injection of AP-5 ( b ), NBQX ( b ), AIP ( e ), and Rpc ( h ) but not of anisomycin ( j ) impaired the preference for the new object as compared to the familiar object at 1-min RTI. With AIP, however, this effect was partial, as the new object was still preferred as compared to 2 of the familiar objects. Pre-study phase injection of AIP ( d ) and Rpc ( g ), as compared to injection of vehicle, significantly reduced the percentage increase (above the vehicle group) of AMPA-R phosphorylation at S831 and S845, respectively, induced 1 min after the exposure to 6 different objects. Post-study phase administration of all treatments fully impaired performance in the 6-DOT at 24-h RTI ( c , i , k ), except for the AIP ( f ), where the new object was preferred

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

View 3 publications 3 publications

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

ELISA (ELISA)

Assay Dependent

Product Specifications

Species Reactivity

Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide derived from the rat GluR1 protein.

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 0.15M NaCl

Contains

no preservative

Storage conditions

-20°C

RRID

AB_2533280

Target Information

GluR1 (glutamate receptor 1) is an ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter, L-glutamate, induces a conformation change that leads to the opening of the cation channel and converts the chemical signal to an electrical impulse. Then, the receptor desensitizes rapidly and enters a transiet inactive state characterized by the presence of a bound agonist. In the presence of CACNG4, CACNG7, or CACNG8 GluR1 shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: AI853806; AMPA-selective glutamate receptor 1; GluA1; GLUH 1; GluR K1; GluR-1; GluR-A; GluR-K1; GluR1; GluRK1; glutamate receptor; Glutamate receptor 1; glutamate receptor A; Glutamate receptor ionotropic, AMPA 1; glutamate receptor subunit GluR1; glutamate receptor, ionotropic, AMPA1 (alpha 1); GPRC1A; Gria 1; GRM1A; HGBR1; HIPA; MGC13325; MGC133252; mGlu1; MGLUR1; MGLUR1A; NMDA1; NMDAR1; OTTHUMP00000160643; OTTHUMP00000165781; OTTHUMP00000224241; OTTHUMP00000224242; OTTHUMP00000224243; RP11-350O14.1

Gene Aliases: GluA1; gluR-A; Glur1; Gria1

UniProt ID: (Rat) P19490

Entrez Gene ID: (Rat) 50592

Molecular Function: ion channel ionotropic glutamate receptor ligand-gated ion channel receptor transporter