Invitrogen

Phospho-GluR1 (Ser845) Polyclonal Antibody

Product Image Gallery

Phospho-GluR1 (Ser845) Antibody — Fig. 3 In high memory load condition object LTM shares the same molecular pathway as object memory at short retention interval with the addition of novel protein synthesis. a Photos of representative Nissl-stained coronal sections of animals bilaterally implanted with permanent cannulae and injected within the hippocampus. b , c , e , f , h , i , j , k Effects of hippocampus focal pre-training and post-training administrations of inhibitors on the preference for the new object ( N ) and compared to familiar objects ( F ) in the test phase of the 6-DOT performed at 1-min and 24-h RTI, respectively. In all cases vehicle-injected animals explored the new object significantly more than all the other familiar objects. Pre-study phase injection of AP-5 ( b ), NBQX ( b ), AIP ( e ), and Rpc ( h ) but not of anisomycin ( j ) impaired the preference for the new object as compared to the familiar object at 1-min RTI. With AIP, however, this effect was partial, as the new object was still preferred as compared to 2 of the familiar objects. Pre-study phase injection of AIP ( d ) and Rpc ( g ), as compared to injection of vehicle, significantly reduced the percentage increase (above the vehicle group) of AMPA-R phosphorylation at S831 and S845, respectively, induced 1 min after the exposure to 6 different objects. Post-study phase administration of all treatments fully impaired performance in the 6-DOT at 24-h RTI ( c , i , k ), except for the AIP ( f ), where the new object was preferred

Phospho-GluR1 (Ser845) Antibody in Western Blot (WB) — Western blot analysis of phospho-GluR1 in rat brain lysate using a phospho-Glur1 pSer845 polyclonal antibody (Product # 36-8300).

Product Details

Tested Applications

Dilution

ELISA (ELISA)

Assay Dependent

Immunofluorescence (IF)

Assay Dependent

Immunohistochemistry (IHC)

1:1000

Western Blot (WB)

1:1000

Published Applications

Western Blot (WB)

See 3 publications below

Immunohistochemistry - Free Floating (IHC (Free))

See 1 publication below

Product Specifications

Species Reactivity

Rat

Published species

Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide derived from the rat GluR1 protein.

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 100µg/mL BSA, 50% glycerol, 0.15M NaCl

Contains

no preservative

Storage conditions

-20°C

RRID

AB_2533280

Target Information

GluR1 (glutamate receptor 1) is an ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter, L-glutamate, induces a conformation change that leads to the opening of the cation channel and converts the chemical signal to an electrical impulse. Then, the receptor desensitizes rapidly and enters a transiet inactive state characterized by the presence of a bound agonist. In the presence of CACNG4, CACNG7, or CACNG8 GluR1 shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: AMPA 1; AMPA-selective glutamate receptor 1; GluA1; GLUH1; GluR-1; GluR-A; GluR-K1; GLUR1; GLURA; glutamate receptor; Glutamate receptor 1; glutamate receptor A; Glutamate receptor ionotropic; Glutamate receptor ionotropic, AMPA 1; glutamate receptor subunit GluR1; glutamate receptor, ionotropic, AMPA1 (alpha 1); GRIA1; HBGR1; ionotropic; MGC133252

Gene Aliases: GluA1; gluR-A; Glur1; Gria1

UniProt ID: (Rat) P19490

Entrez Gene ID: (Rat) 50592

Molecular Function: ion channel ionotropic glutamate receptor ligand-gated ion channel receptor transporter