|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to amino acid sequence within GluR1 which includes phosphorylated Ser831|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.01% BSA, 50% glycerol|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Predicted to react with chicken, dog, human and mouse based on sequence homology.
For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM NaF should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background. PA1-84839 detects a band of ~100kDa in rat brain lysates.
GluR1, widely expressed throughout the nervous system, is a component of AMPA receptors (alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid receptors), which play a key role at excitatory synapses. Phosphorylation of Ser831 and Ser845 is reported to potentiate GluR1 function, suggesting a possible role in synaptic plasticity, learning and memory.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.