|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Sythetic peptide corresponding to amino acid residues surrounding phospho-Ser845 of rat GluR1|
|Storage buffer||HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
This antibody recognizes a ~100kD band corresponding to GluR1 phosphorylated at Ser845 in Western blots of rat brain extracts. Immunolabeling can blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide, and is completely eliminated by treatment with lambda-phosphatase.
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct subunits GluR1-4 and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is also potentiated by phosphorylation at Ser831. In addition, phosphorylation of this site has been linked to synaptic plasticity as well and learning and memory.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
PDE10 inhibition increases GluA1 and CREB phosphorylation and improves spatial and recognition memories in a Huntington's disease mouse model.
PA1-12749 was used in western blot to study the role of increased phosphorylation of GluA1 and CREB in the mechanism by which pharmacological inhibition of PDE10 improves memory in a murine model of Huntington's disease
|Giralt A,Saavedra A,Carretón O,Arumí H,Tyebji S,Alberch J,Pérez-Navarro E||Hippocampus (23:684)||2013|
Environmental novelty activates ß2-adrenergic signaling to prevent the impairment of hippocampal LTP by Aß oligomers.
PA1-12749 was used in western blot to study the role of beta2-adrenergic receptor activation in the mechanism by which environmental enrichment inhibits the deleterious effects of Abeta oligomers on hippocampal function
|Li S,Jin M,Zhang D,Yang T,Koeglsperger T,Fu H,Selkoe DJ||Neuron (77:929)||2013|
Effects of minocycline on cocaine sensitization and phosphorylation of GluR1 receptors in 5-lipoxygenase deficient mice.
PA1-12749 was used in western blot to study the role of 5-lipoxygenase in the effect of antibiotic minocycline on cocaine sensitization
|Chen H,Manev H||Neuropharmacology (60:1058)||2011|