Western blot of rat brain homogenate showing specific immunolabeling of the ~100 kDa GluR2 protein phosphorylated at Ser 880 (control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (peptide) but not by the corresponding non-phosphorylated peptide (not shown).
|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser880 of rat GluR2.|
|Storage buffer||0.01M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 100µg/ml BSA|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene.
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