Immunofluorescent analysis of Phospho-Glucocorticoid Receptor pSer211 in HeLa cells, dexamethasone-treated, using a Phospho-Glucocorticoid Receptor pSer211 polyclonal antibody (Product # PA5-17668) (green). Actin filaments are labeled with a fluorescent red phalloidin.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding serine 211 of human glucocorticoid receptor|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other unrelated phosphorylated proteins.
The protein encoded by this gene is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
BAY 11-7085 induces glucocorticoid receptor activation and autophagy that collaborate with apoptosis to induce human synovial fibroblast cell death.
PA5-17668 was used in western blot to report that glucocorticoid receptor activation and PPAR-gamma inactivation mediate BAY 11-7085-induced autophagy
|Relic B,Charlier E,Deroyer C,Malaise O,Neuville S,Desoroux A,Gillet P,de Seny D,Malaise MG||Oncotarget (7:23370)||2016|