Immunohistochemical analysis of Phospho-Glycogen Synthase pSer640 in formalin-fixed, paraffin-embedded human prostate tissue using a Phospho-Glycogen Synthase pSer640 polyclonal antibody (Product # PA1-26469) at a dilution of 5 µg/ml following heat-mediated antigen retrieval. Staining shows as a precipitated red signal with a hematoxylin purple nuclear counterstain.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide, conjugated to KLH, corresponding to residues A(631) Q G Y R Y P R P A (pS) V P(642) of human muscle Glycogen Synthase.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26469 detects Glycogen Synthase (phospho S640) from human and mouse samples.
PA1-26469 has been successfully used in Western blot procedures.
The PA1-26469 immunogen is a synthetic phosphopeptide, conjugated to KLH, corresponding to residues A(631) Q G Y R Y P R P A (pS) V P(642) of human muscle Glycogen Synthase.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.