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Peptide Competition and Phosphatase Treatment Lysates prepared from 3T3-L1 cells left unstimulated (1), stimulated with Wortmanin for 90 minutes (2), stimulated with Wortmanin for 90 minutes plus insulin for 60 minutes (3), stimulated with insulin for 20
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Glycogen Synthase that contains serine 641 and serine 645. The sequence is conserved in human, mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Accelerated recovery of mitochondrial membrane potential by GSK-3β inactivation affords cardiomyocytes protection from oxidant-induced necrosis.
44-1092G was used in western blot to identify the role of mKATP channel activation in mitochondrial membrane potential and cell death
|Sunaga D,Tanno M,Kuno A,Ishikawa S,Ogasawara M,Yano T,Miki T,Miura T||PloS one (9:null)||2014|
Translocation of glycogen synthase kinase-3β (GSK-3β), a trigger of permeability transition, is kinase activity-dependent and mediated by interaction with voltage-dependent anion channel 2 (VDAC2).
44-1092G was used in western blot to examine the mechanism of translocation in regards to glycogen synthase kinase-3 protein
|Tanno M,Kuno A,Ishikawa S,Miki T,Kouzu H,Yano T,Murase H,Tobisawa T,Ogasawara M,Horio Y,Miura T||The Journal of biological chemistry (289:29285)||2014|
EC 18.104.22.168; glycogen [starch] synthase; Glycogen [starch] synthase, muscle; glycogen synthase; glycogen synthase 1 (muscle); glycogen synthase 3, brain; GYS; muscle
GSY; GYS; GYS1; Gys3; MGS