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Western blot analysis of Phospho-HBP1 pSer402 using Phospho-HBP1 pSer402 polyclonal antibody (Product # PA5-36809) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with PMA (100nM, 15min), Lane 2: Mouse spleen tissue lysate, Lane 3: Rat spleen tissue lysate.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human HBP1 around the phosphorylation site of Serine 402|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 58 and 80 kDa.
Purity is >95% by SDS-PAGE.
The HMG-box protein-1 (HBP1) is a member of the HMG family of transcription factors, which are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, that mediates DNA binding. HBP1 binds to the tumor suppressor proteins Rb and p130 and initiates cell cycle arrest. Terminal cell differentiation requires this initial cell cycle arrest followed by the coordinated expression of genes defined as tissue-specifc markers. Along with initiating the commitment to cell differentiation, the continued activity of HBP1 abrogates the expression of tissue-specific genes by associating with the MyoD proteins. In muscle cell differentiation, the MyoD family of transcription factors, which include Myf5, MyoD and myogenein, induce the expression of these cell-type specific proteins and contribute to the development of cell phenotypes. The progression of terminal differentiation is, therefore, dependent on both a decrease in HBP1 activity and the corresponding activation of MyoD-induced gene transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
HBP1; high mobility group box transcription factor 1; high mobility group protein 1; HMG box transcription factor 1; HMG box-containing protein 1; HMG-box containing protein 1
1700058O05Rik; BE629963; C330012F01Rik; C86454; HBP1; Hmgb1