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Phospho-HCK (Tyr522) Antibody (PA5-105481) in WB
Western blot analysis of Phospho-HCK (Tyr522) in extracts from various samples. Samples were incubated with Phospho-HCK (Tyr522) polyclonal antibody (Product # PA5-105481). Lane 1: Serum starvation treated RAW264.7 cells, blocked with antigen-specific peptides; Lane 2: Serum starvation treated RAW264.7 cells; Lane 3: Heat-shock treated HepG2 cells.
Product Details
PA5-105481
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human HCK(Accession P08631), corresponding to amino acid residues around phosphorylated Tyr522.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2816909
Product Specific Information
Antibody detects endogenous levels of Hck only when phosphorylated at Tyr522.
Target Information
Hematopoietic cell kinase (HCK) is a SRC-related kinase expressed in lymphoid and hematopoietic cells and may play a role in some forms of leukemia. (HCK Alias: DKFZp686D0638). HCK is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B cell/myeloid kinase; B-cell/myeloid kinase; BMK; Hematopoietic cell kinase; Hemopoietic cell kinase; p56-HCK/p59-HCK; p59-HCK/p60-HCK; p59Hck; p61Hck; Tyrosine-protein kinase HCK
Gene Aliases: AI849071; Bmk; HCK; JTK9; p59Hck; p61Hck
UniProt ID: (Human) P08631, (Mouse) P08103
Entrez Gene ID: (Human) 3055, (Mouse) 15162
leukocyte migration involved in immune response
innate immune response-activating signal transduction
protein phosphorylation
inflammatory response
cell adhesion
transmembrane receptor protein tyrosine kinase signaling pathway
integrin-mediated signaling pathway
mesoderm development
positive regulation of cell proliferation
regulation of cell shape
viral process
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
cell differentiation
positive regulation of actin filament polymerization
lipopolysaccharide-mediated signaling pathway
peptidyl-tyrosine autophosphorylation
Fc-gamma receptor signaling pathway involved in phagocytosis
negative regulation of apoptotic process
leukocyte degranulation
respiratory burst after phagocytosis
protein autophosphorylation
regulation of defense response to virus by virus
regulation of inflammatory response
regulation of phagocytosis
regulation of sequence-specific DNA binding transcription factor activity
interferon-gamma-mediated signaling pathway
regulation of podosome assembly
positive regulation of actin cytoskeleton reorganization
immune system process
exocytosis
phagocytosis
phosphorylation
regulation of cell proliferation
innate immune response
defense response to Gram-positive bacterium
cellular response to peptide hormone stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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