Western blot analysis of phospho HDAC7 in (A) recombinant fusion protein containing a phosphorylated serine at position 318 and (B) fusion protein containing an unphosphorylated serine at position 318, using a Phospho-HDAC-7 pSer318 polyclonal antibody (Product # PA5-23203) at 0.1 ug/ml.
|Tested species reactivity||Bovine, Dog, Horse, Human, Non-human primate, Zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (313 CLSRTRSEPLP 322) of Phospho-HDAC-7 pSer318 protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.1-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Histone deacetylase 7 is a thymus-specific, intracellular, transcription factor belonging to the class IIa histone deacetylase family. Reports suggest that dephosphorylation and nuclear localization of HDAC7 is promoted by Myosin phosphatase, thus regulating the repression of Nur77 and inhibition of apoptosis in CD4+CD8+ double-positive thymocytes. HDAC7 plays a specific role in maintaining vascular integrity by repressing the expression of matrix metalloproteinase (MMP) 10. It promotes repression mediated by transcriptional corepressor NCOR2 and is an efficient corepressor of the androgen receptor (AR). It is also responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.