Invitrogen

Phospho-HDAC6 (Ser22) Polyclonal Antibody

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Antibody Testing Data (4)

Phospho-HDAC6 (Ser22) Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

Phospho-HDAC6 (Ser22) Antibody (PA5-105035) in ICC/IF

Immunofluorescent analysis of Phospho-HDAC6 (Ser22) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-HDAC6 (Ser22) polyclonal antibody (Product # PA5-105035) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 ... View More

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Phospho-HDAC6 (Ser22) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Phospho-HDAC6 (Ser22) in HeLa cells(4h of LPS treatment). Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Phospho-HDAC6 (Ser22) polyclonal antibody (Product # PA5-105035) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

Phospho-HDAC6 (Ser22) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Phospho-HDAC6 (Ser22) in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% saponin, blocked with 10% serum (45 min, 37°C) incubated with Phospho-HDAC6 (Ser22) polyclonal antibody (Product # PA5-105035) using a dilution of 1:400 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Phospho-HDAC6 (Ser22) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Phospho-HDAC6 (Ser22) in human testis tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-HDAC6 (Ser22) polyclonal antibody (Product # PA5-105035) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Phospho-HDAC6 (Ser22) Antibody in Western Blot (WB) — Western blot analysis of Phospho-HDAC6 (Ser22) in NIH-3T3 cell lysate (right lane: treated with the antigen-specific peptide). Samples were incubated with Phospho-HDAC6 (Ser22) polyclonal antibody (Product # PA5-105035).

Product Details

PA5-105035

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:100-1:500

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human HDAC6 around the phosphorylation site of Ser22.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2816508

Target Information

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ16239; HD6; HDA6; histone deacetylase 5; Histone deacetylase 6; Histone deacetylase mHDA2; protein phosphatase 1, regulatory subunit 90; scurfy candidate 6; Tubulin-lysine deacetylase HDAC6

Gene Aliases: CPBHM; HD6; Hdac5; HDAC6; JM21; KIAA0901; mHDA2; PPP1R90; Sfc6

UniProt ID: (Human) Q9UBN7, (Mouse) Q9Z2V5

Entrez Gene ID: (Human) 10013, (Mouse) 15185

Molecular Function: chromatin/chromatin-binding, or -regulatory protein histone modifying enzyme

protein polyubiquitination transcription, DNA-templated protein deacetylation misfolded or incompletely synthesized protein catabolic process intracellular protein transport organelle organization negative regulation of microtubule depolymerization response to toxic substance positive regulation of signal transduction response to organic substance regulation of receptor activity regulation of autophagy positive regulation of epithelial cell migration negative regulation of hydrogen peroxide metabolic process positive regulation of receptor biosynthetic process macroautophagy histone deacetylation regulation of protein stability lysosome localization peptidyl-lysine deacetylation cellular response to topologically incorrect protein regulation of gene expression, epigenetic ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway protein complex disassembly negative regulation of protein complex disassembly regulation of fat cell differentiation negative regulation of proteolysis negative regulation of transcription, DNA-templated negative regulation of oxidoreductase activity mitochondrion localization response to misfolded protein regulation of microtubule-based movement regulation of androgen receptor signaling pathway regulation of establishment of protein localization cellular response to hydrogen peroxide aggresome assembly polyubiquitinated misfolded protein transport Hsp90 deacetylation response to growth factor histone H3 deacetylation cellular response to misfolded protein positive regulation of chaperone-mediated protein complex assembly tubulin deacetylation mitophagy in response to mitochondrial depolarization positive regulation of hydrogen peroxide-mediated programmed cell death regulation of mitophagy regulation of transcription, DNA-templated ubiquitin-dependent protein catabolic process chromatin modification

Process(es)

core promoter binding actin binding histone deacetylase activity protein binding beta-catenin binding microtubule binding zinc ion binding enzyme binding polyubiquitin binding ubiquitin protein ligase binding NAD-dependent histone deacetylase activity (H3-K14 specific) histone deacetylase binding tubulin deacetylase activity alpha-tubulin binding tau protein binding beta-tubulin binding misfolded protein binding Hsp90 protein binding dynein complex binding hydrolase activity histone deacetylase activity (H3-K14 specific) histone deacetylase activity (H3-K9 specific) histone deacetylase activity (H4-K16 specific) ubiquitin binding metal ion binding NAD-dependent histone deacetylase activity (H3-K9 specific) NAD-dependent histone deacetylase activity (H4-K16 specific) NAD-dependent histone deacetylase activity (H3-K18 specific)