Immunofluorescent analysis of Phospho-HP1-gamma pSer83 in HeLa cells, forskolin and IBMX-treated (center), using a Phospho-HP1-gamma pSer83 polyclonal antibody (Product # PA5-17210) (green). Actin filaments are labeled with a fluorescent red phalloidin.
|Tested species reactivity||Bovine, Fruit fly, Horse, Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to amino acids surrounding pSer83 of human HP1 gamma.|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with HP1-alpha or HP1-beta proteins.
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.