Immunofluorescent analysis of Phospho-HSL pSer563 in 3T3-L1 adipocytes, isoproterenol-treated, using a Phospho-HSL pSer563 polyclonal antibody (Product # PA5-17488) (red). Lipid droplets have been labeled with a BODIPY fluorescent dye (green). DNA is labeled using a fluorescent blue dye.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pSer552 of human HSL (equivalent to pSer563 of rat HSL)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with Ser565 phosphorylated HSL.
The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Extramitochondrial OPA1 and adrenocortical function.
PA5-17488 was used in western blot to study extra-mitochondrial OPA1 and its potential biological function in adrenocortical cells
|Fülöp L,Rajki A,Katona D,Szanda G,Spät A||Molecular and cellular endocrinology (381:70)||2013|