Western blot analysis of HSP27 (Ser78) in recombinant fusion protein containing 1) a phosphorylated serine at position 78 and 2) an unphosphorylated serine at position 78, using a Phospho-HSP27 pSer78 polyclonal antibody (Product # PA5-23340) at 0.01 ug/ml.
|Tested species reactivity||Dog, Horse, Human, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (73 YSRAL(S)RQLSS 83) of Phospho-HSP27 pSer78 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.01-0.1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
HSP27, also known as Heat shock 27 kDa protein, is a cytoplasmic protein in interphase cells and colocalizes with mitotic spindles in mitotic cells. It translocates to the nucleus during heat shock. HSP27 belongs to the small heat shock protein (HSP20) family and is known to be an important protein in stress resistance and actin organization and associates with alpha- and beta-tubulin, microtubules and CRYAB. It also interacts with other heat shock proteins like HSPB8 and HSPBAP1. HSP27 is expressed in response to environmental stresses such as heat shock, or estrogen stimulation in MCF-7 cells. Ubiquitous expression is seen in most tissues with highest levels in heart and in tissues composed of striated and smooth muscle. Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuronopathy type 2B (HMN2B).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.