Western blot analysis of Phospho-Histone H2A.X (Ser139) in HeLa whole cell lysate (Lane 1) and HeLa treated with UV light for 2 hrs (Lane 2). Samples were loaded at 20µg per lane onto a 15% SDS-polyacrylamide gel, transferred to a PVDF membrane (semi-dry) and blocked with TBS containing TBST and 5% non-fat dry milk for 2 hrs at room temperature. The membrane was washed in TBST and probed with an anti-Phospho-Histone H2A.X polyclonal antibody (Product # LF-PA0025) at a dilution of 1:5000 overnight at 4ºC and washed in TBST. Secondary detection was performed using a Goat anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, HRP conjugate (Product # 31462) at a dilution of 1:5000, followed by ECL detection. Data provided courtesy of Antibody Resource.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is HeLa cells treated with camptothecin.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.