Immunofluorescent analysis of Phospho-Histone H3 (pSer10, green) in human uveal melanoma cells. Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature, and blocked with 5% BSA for 1 hour at room temperature. Cells were probed with a Phospho-Histone H3 (pSer10) monoclonal antibody (Product # MA5-15220) at a dilution of 1:600 for 1 hour at room temperature, washed with PBS, and incubated with an Alexa-Fluor 488-conjugated anti-mouse IgG secondary antibody at a dilution of 1:500 for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33258. Images were taken on a fluorescent microscope at 20X magnification. Data courtesy of the Innovators Program.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pSer10 of human histone H3|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:25|
|Immunohistochemistry (Frozen) (IHC (F))||1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other phosphorylated histones or acetylated histone H3.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.