Immunohistochemical analysis of Phospho-Histone H3 pSer10+acetyl Lys9 in formalin-fixed, paraffin-embedded mouse testis tissue using a Phospho-Histone H3 pSer10+acetyl Lys9 polyclonal antibody (Product # PA1-25462) at a dilution of 0.4 µg/ml.
|Tested species reactivity||Bovine, Human, Mouse, Rat, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic, acetylated and phosphorylated (acetyl K9 and phospho S10) peptide, conjugated to KLH, corresponding to the N-terminal residues 7-20 of human Histone H3.|
|Contains||15mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25462 detects Histone H3 (phospho S10 acetyl K9) from human samples. PA1-25462 is expected to cross react with mouse, rat, Xenopus laevis, bovine, C. elegans, chicken, Drosophilia melanogaster, and a wide range of other species due to sequence homology
PA1-25462 has been successfully used in immunofluorescence and Western blot procedures.
The PA1-25462 immunogen is a synthetic, acetylated and phosphorylated (acetyl K9 and phospho S10) peptide, conjugated to KLH, corresponding to the N-terminal residues 7-20 of human Histone H3.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.