Histone extracts of HeLa cells (15 µg) were analyzed by western blot using the anti-H3K9acS10p crude serum (Cat. no. 49-1011), diluted 1:250 in TBS-Tween containing 5% milk. The position of the protein of interest is indicated on the left; the marker (in kDa) is shown on the right.
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Raised against histone H3 containing the acylated lysine 9 and the phosphorylated serine 10 (H3K9acS10p), using a KLHconjugated synthetic peptide.|
|Storage buffer||whole serum|
|Contains||<0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||15 ug|
|ELISA (ELISA)||1:1,000 to 1:4,000|
|Western Blot (WB)||1:250|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ChIP assay (ChIP)||See 1 publications below|
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post tranlationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Epigenetic drift towards histone modifications regulates CAV1 gene expression in colon cancer.
49-1011 was used in ChIP assay to analyze epigenetic drift towards histone modifications and how they regulate CAV1 gene expression in colon cancer
|Deb M,Sengupta D,Kar S,Rath SK,Roy S,Das G,Patra SK||Gene (581:75)||2016|