Western blot analysis of Phospho-Hormone-Sensitive Lipase pSer554/pSer855 in Adriamycin-treated HeLa whole cell lysates with a blocking peptide (lane 1) and no blocking peptide (lane 2) using a Phospho-Hormone-Sensitive Lipase pSer554/pSer855 polyclonal antibody (Product # PA5-35493). This image shows detection of the HSL isoform 2, which has a predicted molecular weight of 84.1kDa.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthesized peptide derived from human HSL around the phosphorylation site of Serine 855/554|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.5, with 50% glycerol|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
This antibody detects isoform 1 (phospho-HSL pSer855, predicted molecular weight 116.6kDa) and isoform 2 (phospho-HSL pSer554, predicted molecular weight 84.1kDa).
In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.