Rockland

Phospho-Huntington (Ser421) Polyclonal Antibody

View all (46) Huntingtin antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Phospho-Huntington (Ser421) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Rocklands Affinity Purified anti-Huntingtin pS421 antibody was used at a 1:100 dilution to detect phosphorylated Huntingtin by immunohistochemistry in human brain cerebellum. Positive cytoplasmic staining is observed in neurons. Tissue was formalin-fixed and paraffin embedded. Personal Communication,

Phospho-Huntington (Ser421) Antibody in Western Blot (WB) — Western blot analysis after AKT and phosphatase treatment is shown using Rocklands Affinity Purified anti-Huntingtin pS421 antibody. In A) untreated lysates from N2A and 293A cells were stained directly using anti-Huntingtin pS421 antibody. Full length staining of Huntingtin is noted, albeit at low levels of expression, as well as a strongly staining band at 200 kDa that may represent staining of truncated protein. In B) staining is shown after immunoprecipitation using a monoclonal antibody (Mab2166) followed by AKT treatment (to phosphorylate), along with untreated, and phosphatase (PP) treated (dephosphorylate) immunoprecipitated Htt. Full length phosphorylated huntingtin is clearly detected in these immunopurified samples (except dephosphorylated). In C) lysates are treated directly with AKT or PP to alter the phosphorylation status of Htt. Personal communication, Simon Warby, CMMT, Vancouver, BC.

Product Details

600-401-433

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500- 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

ELISA (ELISA)

1:10,000-1:40,000

Immunoprecipitation (IP)

1:500-1:3,000

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Huntingtin pS421 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 400-425 of Human Huntington Disease Protein.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.96 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is specific for phosphorylated human Huntington protein at the pS421 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Huntington homologues from chimpanzee, pig and chicken. Cross reactivity with Huntington protein homologues from mouse and rat may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Huntington protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.

Target Information

Huntingtin is a disease gene linked to Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. HD is a mid-life onset autosomal dominant neurodegenerative disease that is characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10-20 years.The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: HD protein; Huntingtin; Huntingtin, myristoylated N-terminal fragment; Huntington disease protein

Gene Aliases: HD; HTT; IT15

UniProt ID: (Human) P42858

Entrez Gene ID: (Human) 3064

Function(s)

establishment of mitotic spindle orientation retrograde vesicle-mediated transport, Golgi to ER apoptotic process Golgi organization central nervous system development positive regulation of inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity regulation of protein phosphatase type 2A activity vocal learning positive regulation of cilium assembly vesicle transport along microtubule animal organ development mRNA transport negative regulation of cysteine-type endopeptidase activity negative regulation of extrinsic apoptotic signaling pathway

Process(es)

p53 binding receptor binding protein binding profilin binding transcription factor binding dynactin binding identical protein binding ion channel binding dynein intermediate chain binding beta-tubulin binding