Western blot analysis of Phospho-I-kappa-B-alpha pSer32+Ser36 in COS7 whole cell lysates with a blocking peptide (lane 1) and no blocking peptide (lane 2) using a Phospho-I-kappa-B-alpha pSer32+Ser36 polyclonal antibody (Product # PA5-35846).
|Tested species reactivity||Non-human primate, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from human I kappaB- alpha around the phosphorylation site of Serine 32/Serine 36|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Nucleoside, nucleotide and nucleic acid metabolism mRNA transcription mRNA transcription regulation Signal transduction Intracellular signaling cascade NF-kappaB cascade Intracellular protein traffic Immunity and defense Stress response Apoptosis Cell proliferation and differentiation