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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human IkBa that contains serines 32 and 36. The sequence is conserved in mouse and pig.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Acrolein inhibits cytokine gene expression by alkylating cysteine and arginine residues in the NF-kappaB1 DNA binding domain.
||Lambert C,Li J,Jonscher K,Yang TC,Reigan P,Quintana M,Harvey J,Freed BM||The Journal of biological chemistry (282:19666)||2007|
I-kappa-B-alpha, IkappaBalpha, NF-kappa-B inhibitor alpha, ikB-alpha, major histocompatibility complex enhancer-binding protein MAD3, nuclear factor of kappa light chain gene enhancer in B-cells, IKBA, MAD3, NF-kappaB inhibitor alpha, NFKBI, NFKBIA
IKBA, NFKBI, MAD-3
Nucleoside, nucleotide and nucleic acid metabolism mRNA transcription mRNA transcription regulation Signal transduction Intracellular signaling cascade NF-kappaB cascade Intracellular protein traffic Immunity and defense Stress response Apoptosis Cell proliferation and differentiation