Western blot analysis of Phospho-I-kappa-B-beta pThr19 using Phospho-I-kappa-B-beta pThr19 polyclonal antibody (Product # PA5-36772) at a dilution of 1:500. Lane 1: Hela cell lysate treated with TNF-alpha (20ng/ml, 15min), Lane 2: NIH-3T3 cell lysate treated with TNF-alpha (20ng/ml, 15min), Lane 3: PC12 cell lysate treated with TNF-alpha (20ng/ml, 15min).
|Tested species reactivity||Mouse, Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human IkB-beta around the phosphorylation site of Threonine 19|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 48 kDa.
Purity is >95% by SDS-PAGE.
The NF-kB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkB proteins. Most agents that activate NF-kB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkB. The key regulatory step in this pathway involves activation of a high molecular weight IkB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKK alpha and IKK beta serve as the catalytic subunits of the kinase and IKK gamma serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKK beta (Ser176 and Ser180 in IKK alpha), which causes conformational changes, resulting in kinase activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.