|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to a portion of IR/IGF1R containing tyrosines 1158, 1162, and 1163 of human insulin receptor.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26725 detects Insulin Receptor / IGF1R (phospho Y1158 + Y1162 + Y1163) from human and mouse samples. PA1-26725 is expected to cross react with rat (100% conserved) due to sequence homology.
PA1-26725 has been successfully used in Western blot procedures.
The PA1-26725 immunogen is a synthetic phosphopeptide corresponding to a portion of IR/IGF1R containing tyrosines 1158, 1162, and 1163 of human insulin receptor (tyrosines 1131, 1135 and 1136 in IGF1R).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.