Western blot analysis of Phospho-IGF1R pTyr1165+pTyr1166 in insulin-treated 293 whole cell lysates with a blocking peptide (lane 1) and no blocking peptide (lane 2) using a Phospho-IGF1R pTyr1165+pTyr1166 polyclonal antibody (Product # PA5-35452). This image shows reactivity with the cleaved beta chain of IGF1R (aa 741-1367).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthesized peptide derived from human IGF1R around the phosphorylation site of Tyrosine 1165/Tyrosine 1166|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.5, with 50% glycerol|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
This antibody reacts with the beta chain of IGF1R.
Insulin growth factor 1 receptor belongs to the family of tyrosine receptor kinases. It is involved in activation of various substrates including IGF and neurotensin, regulation of metabolism, cell division, maintenance, and inflammation response. The protein is a tetramer consisting of two alpha, and two beta subunits. The alpha-subunits binds to insulin growth factor 1 with higher affinity than to insulin growth factor 2. Binding results in a conformational change, followed by autophosphorylation of IGF-1R at positions 1134, 1135, 1136. The activated IGF-1R in turn, causes phosphorylation of substrate proteins, followed by sequential activation of RAS, RAF, and mitogen-activated protein kinase isoforms ERK, p38, and JNK, leading to the transcription of genes that drive proliferation. This antibody recognizes the beta-subunit of IGF-1R.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein metabolism and modification Protein modification Protein phosphorylation Signal transduction Cell surface receptor mediated signal transduction Receptor protein tyrosine kinase signaling pathway Developmental processes Embryogenesis Cell cycle Cell cycle control Apoptosis Inhibition of apoptosis Cell proliferation and differentiation