Invitrogen

Phospho-IKK alpha/beta (Ser176, Ser180) Recombinant Polyclonal Antibody (7HCLC)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

1 Reference

View all (16) IKK alpha/beta antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Phospho-IKK alpha/beta (Ser176, Ser180) Antibody in Immunocytochemistry (ICC/IF) — Time course showing induction of the TNF-a signaling cascade upon treatment: Cellular localization of proteins in the NF-KB signaling pathway was detected upon treatment of HeLa cells with TNF alpha (50 ng/mL) for 5, 10 and 25 min. Fixed and permeabilized cells were stained with Anti-IKK total Recombinant Rabbit Polyclonal Antibody (Product # 710719, 1 µg/mL) or Anti-IKK alpha/beta (pS176/pS180) Recombinant Rabbit Polyclonal Antibody (Product # 710676, 1 µg/mL) or Anti-NF-kB Recombinant Rabbit Polyclonal Antibody (Product # 710048, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Images showing cytoplasmic staining of IKK total and NF-kB (panel a & e; green) in untreated cells. No significant basal levels of phospho-IKK alpha/beta (panel i; green) were detected. Treatment with TNF-a led to a significant increase in the levels of IKK (panel b-d; green) and IKK alpha/beta (pS176/pS180) (panel j-l; green) in the cytosol, specifically at the perinucleus, and a corresponding translocation of NF-KB to the nucleus (panel f-h; green). No background staining was observed in control cells with no primary antibody (panel m).The nuclei (blue) were stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938, 1:50). Cytoskeletal F-actin staining (red) was done using Alexa Fluor® 594 Phalloidin (Product # A12381, 1:200).

Phospho-IKK alpha/beta (Ser176, Ser180) Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A decrease of intensity was observed in TNF alpha induced phosphorylation of IKK alpha/beta in IKK alpha knockout (KO) cell line compared to control cell lineusing Anti-Phospho-IKK alpha/beta (pS176pS180) Recombinant Rabbit Polyclonal Antibody (Product # 710676).

Product Details

710676

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1-2 µg/mL

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rabbit / IgG

Expression System

EXPI293

Class

Recombinant Polyclonal

Type

Antibody

Clone

7HCLC

Immunogen

phosphopeptide corresponding to human IKK alpha (Ser176/180)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2532752

Product Specific Information

This antibody is predicted to react with Dog, Rabbit, Pig, Mouse, Rat, Bovine and Goat.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

IKK alpha/beta is encoded by CHUK (conserved helix-loop-helix ubiquitous kinase) and IKKB (inhibitor of kappa light polypeptide gene enhancer in B-cells) gene located in chromosome 10 and 8 respectively in humans. Upon stimulation by agents that activate the NF-kB pathway such as TNF alpha, the two subunits of IKK complex, IKK alpha and IKK -beta alongside a regulator NEMO (IKK gamma) phosphorylates IkB proteins. This targets the later for ubiquitin mediated degradation and phosphorylation of NF-kB leading to subsequent translocation to the nucleus. Phosphorylation at serine 176 and 180 located in the activation loop causes a confirmation change that is required for the activation of the kinases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Linear ubiquitination induces NEMO phase separation to activate NF-κB signaling.

Life science alliance

Goel S,Oliva R,Jeganathan S,Bader V,Krause LJ,Kriegler S,Stender ID,Christine CW,Nakamura K,Hoffmann JE,Winter R,Tatzelt J,Winklhofer KF

710676 was used in Western Blotting to show that M1-ubiquitin chains induce phase separation of NEMO and the formation of NEMO assemblies in cells after exposure to IL-1β.

Sat Apr 01 00:00:00 EDT 2023

Bioinformatics

Protein Aliases: Conserved helix-loop-helix ubiquitous kinase; EC 2.7.11.10; I-kappa-B kinase 1; I-kappa-B kinase 2; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; I-kappa-B-kinase beta; IkappaB kinase; IkB kinase alpha subunit; IKK-a kinase; IKK-B; IKK1; IKK2; Inhibitor of nuclear factor kappa-B kinase subunit alpha; Inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKA; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase beta; Nuclear factor NFkappaB inhibitor kinase alpha; Serine/threonine protein kinase IKBKB; TCF-16; TCF17; Transcription factor 16

Gene Aliases: CHUK; IKBKA; IKBKB; IKK-alpha; IKK-beta; IKK1; IKK2; IKKA; IKKB; IMD15; NFKBIKA; NFKBIKB; TCF16

UniProt ID: (Human) O15111, (Human) O14920

Entrez Gene ID: (Human) 1147, (Human) 3551

Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme