Invitrogen

Phospho-IKK alpha/beta (Ser180, Ser181) Polyclonal Antibody

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Phospho-IKK alpha/beta (Ser180, Ser181) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Phospho-IKK alpha/beta (Ser180, Ser181) in HepG2. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with Phospho-IKK alpha/beta (Ser180, Ser181) polyclonal antibody (Product # PA5-104696) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Phospho-IKK alpha/beta (Ser180, Ser181) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Phospho-IKK alpha/beta (Ser180, Ser181) in human gastric cancer tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-IKK alpha/beta (Ser180, Ser181) polyclonal antibody (Product # PA5-104696) using a dilution of 1:200 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Phospho-IKK alpha/beta (Ser180, Ser181) Antibody in Western Blot (WB) — Western blot analysis of Phospho-IKK alpha/beta (Ser180, Ser181) in TNF treated HepG2 cell lysate (right lane: treated with the antigen-specific peptide). Samples were incubated with Phospho-IKK alpha/beta (Ser180, Ser181) polyclonal antibody (Product # PA5-104696).

Product Details

PA5-104696

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human IKK- alpha/ beta around the phosphorylation site of Ser180/181. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	sequential chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2816169

Target Information

IKK alpha/beta is encoded by CHUK (conserved helix-loop-helix ubiquitous kinase) and IKKB (inhibitor of kappa light polypeptide gene enhancer in B-cells) gene located in chromosome 10 and 8 respectively in humans. Upon stimulation by agents that activate the NF-kB pathway such as TNF alpha, the two subunits of IKK complex, IKK alpha and IKK -beta alongside a regulator NEMO (IKK gamma) phosphorylates IkB proteins. This targets the later for ubiquitin mediated degradation and phosphorylation of NF-kB leading to subsequent translocation to the nucleus. Phosphorylation at serine 176 and 180 located in the activation loop causes a confirmation change that is required for the activation of the kinases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Study on Toll-Like Receptor 2-Mediated Inflammation-Induced Familial Hypertension Combined with Hyperlipemia and Its Mechanism.

Journal of healthcare engineering

Liu J,Li C,Wang Q,Hu H,Li C,Qian J

PA5-104696 was used in Western Blot to investigate the influence of Toll-like receptor 2- (TLR2-) mediated inflammation on the occurrence and development of familial hypertension combined with hyperlipemia and its related mechanism.

Thu Apr 21 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: Conserved helix-loop-helix ubiquitous kinase; EC 2.7.11.10; I kappa B kinase 1; I-kappa-B kinase 1; I-kappa-B kinase 2; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; I-kappa-B-kinase beta; IkappaB kinase; IkappaB kinase alpha; IkB kinase alpha subunit; IKK alpha; IKK-A; IKK-a kinase; IKK-B; IKK-beta; IKK1; IKK2; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of kappaB kinase beta; inhibitor of nuclear factor kappa B kinase beta subunit; Inhibitor of nuclear factor kappa-B kinase subunit alpha; Inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKA; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase beta; Nuclear factor NFkappaB inhibitor kinase alpha; Serine/threonine protein kinase IKBKB; TCF-16; TCF17; Transcription factor 16

Gene Aliases: AI132552; AI256658; AIM-1; CHUK; Chuk1; Fbx24; Fbxo24; IKBKA; IKBKB; IKK-2; IKK-alpha; IKK-beta; IKK1; IKK2; IKK[b]; IKKA; IKKB; IKKbeta; IMD15; NFKBIKA; NFKBIKB; TCF16

UniProt ID: (Human) O15111, (Human) O14920, (Mouse) O88351, (Rat) Q9QY78

Entrez Gene ID: (Human) 1147, (Mouse) 12675, (Rat) 309361, (Human) 3551, (Mouse) 16150, (Rat) 84351

Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme