Invitrogen

Phospho-IKK alpha/beta (Ser180, Ser181) Polyclonal Antibody

View all (16) IKK alpha/beta antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

PA5-36718

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide derived from human IKK alpha/ beta around the phosphorylation site of Serine 180/181

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2553677

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 85 kDa (alpha) and 87 kDa (beta).

Purity is >95% by SDS-PAGE.

Target Information

IKK alpha/beta is encoded by CHUK (conserved helix-loop-helix ubiquitous kinase) and IKKB (inhibitor of kappa light polypeptide gene enhancer in B-cells) gene located in chromosome 10 and 8 respectively in humans. Upon stimulation by agents that activate the NF-kB pathway such as TNF alpha, the two subunits of IKK complex, IKK alpha and IKK -beta alongside a regulator NEMO (IKK gamma) phosphorylates IkB proteins. This targets the later for ubiquitin mediated degradation and phosphorylation of NF-kB leading to subsequent translocation to the nucleus. Phosphorylation at serine 176 and 180 located in the activation loop causes a confirmation change that is required for the activation of the kinases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Conserved helix-loop-helix ubiquitous kinase; EC 2.7.11.10; I kappa B kinase 1; I-kappa-B kinase 1; I-kappa-B kinase 2; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; I-kappa-B-kinase beta; IkappaB kinase; IkappaB kinase alpha; IkB kinase alpha subunit; IKK alpha; IKK-1; IKK-2; IKK-A; IKK-a kinase; IKK-B; IKK-beta; IKK2; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of kappaB kinase beta; inhibitor of nuclear factor kappa B kinase beta subunit; Inhibitor of nuclear factor kappa-B kinase subunit alpha; Inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKA; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase beta; Nuclear factor NFkappaB inhibitor kinase alpha; Serine/threonine protein kinase IKBKB; TCF-16; TCF17; Transcription factor 16

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Gene Aliases: AI132552; AI256658; AIM-1; CHUK; Chuk1; Fbx24; Fbxo24; IKBKA; IKBKB; IKK-2; IKK-alpha; IKK-beta; IKK1; IKK2; IKK[b]; IKKA; IKKB; IKKbeta; IMD15; NFKBIKA; NFKBIKB; TCF16

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UniProt ID: (Human) O15111, (Human) O14920, (Mouse) O88351, (Rat) Q9QY78

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Entrez Gene ID: (Human) 1147, (Mouse) 12675, (Rat) 309361, (Human) 3551, (Mouse) 16150, (Rat) 84351

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Function(s)
protein kinase activity protein binding ATP binding IkappaB kinase activity protein homodimerization activity protein heterodimerization activity scaffold protein binding nucleotide binding protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding non-receptor serine/threonine protein kinase protein modifying enzyme
Process(es)
stimulatory C-type lectin receptor signaling pathway skeletal muscle contraction protein phosphorylation inflammatory response immune response I-kappaB kinase/NF-kappaB signaling I-kappaB phosphorylation Rho protein signal transduction response to virus response to toxic substance anatomical structure morphogenesis response to acetate regulation of tumor necrosis factor-mediated signaling pathway response to lipopolysaccharide response to hydroperoxide TRIF-dependent toll-like receptor signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway response to drug positive regulation of I-kappaB kinase/NF-kappaB signaling response to amino acid innate immune response positive regulation of transcription from RNA polymerase II promoter T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity striated muscle cell differentiation stress-activated MAPK cascade nucleotide-binding oligomerization domain containing signaling pathway cellular response to tumor necrosis factor morphogenesis of an epithelial sheet lactation response to organic substance phosphorylation signal transduction by protein phosphorylation osteoclast differentiation mammary gland epithelial cell proliferation response to muscle stretch odontogenesis of dentin-containing tooth mammary gland alveolus development positive regulation of cell proliferation positive regulation of neuron projection development cortical actin cytoskeleton organization neuron projection development tumor necrosis factor-mediated signaling pathway negative regulation of myosin-light-chain-phosphatase activity regulation of phosphorylation serine phosphorylation of STAT protein negative regulation of apoptotic process positive regulation of transcription, DNA-templated interleukin-1-mediated signaling pathway response to fatty acid establishment of protein localization to plasma membrane positive regulation of neuron death regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly B cell homeostasis positive regulation of sodium ion transport positive regulation of cation channel activity
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