Western blot analysis of Phospho-IL-8R beta/CXCR2 pSer347 in PMA-treated NIH-3T3 whole cell lysates with a blocking peptide (lane 1) and no blocking peptide (lane 2) using a Phospho-IL-8R beta/CXCR2 pSer347 polyclonal antibody (Product # PA5-35693).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from human IL-8R beta/CDw128 beta around the phosphorylation site of Serine 347|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Signal transduction Cell surface receptor mediated signal transduction G-protein mediated signaling Immunity and defense Granulocyte-mediated immunity Cytokine/chemokine mediated immunity Cell structure and motility Cell motility