|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of Serine 246(I-F-S(p)-H-P) derived from Human ILK.|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
A suggested positive control for IHC is human brain tissue.
Integrin-linked kinase (ILK) is an adaptor and signaling protein that couples integrins to the actin cytoskeleton and coordinates signal transduction from extracellular matrix and growth factors. By interacting with the focal adhesion proteins PINCH, paxillin, and parvin, ILK regulates integrin-mediated cell adhesion and cytoskeletal dynamics within focal adhesions to regulate cell adhesion, spreading, and migration. ILK is involved in the suppression of apoptosis and promotion of cell survival through the phosphorylation of AKT (Ser473), GSK-3 (Ser9), myosin light chain (Ser18/19), and affixin. ILK activity is stimulated by PI3 kinase and negatively regulated by the tumor suppressor PTEN.
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Protein Aliases: 59 kDa serine/threonine-protein kinase; epididymis secretory protein Li 28; ILK-1; ILK-2; ILK1; ILK2; integrin binding protein kinase; integrin linked kinase; integrin-linked kinase; integrin-linked kinase-2; Integrin-linked protein kinase; P59; p59ILK
Gene Aliases: AA511515; ESTM24; HEL-S-28; ILK; ILK-1; ILK-2; ILK1; ILK2; P59; p59ILK
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