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Immunofluorescence analysis of IR/IGF1R [pY1158] was done on 70% confluent log phase MCF7 cells treated with insulin (100nM for 5 min). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with IR/IGF1R [pY1158] Rabbit polyclonal Antibody (44802G) at 2µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing membrane localization. Panel e shows untreated MCF7 cells. Panel f shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Hamster, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from the region of IR/IGF1R that contains tyrosine 1158 of the human insulin receptor (IR)|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Mouse||Not Cited||Leukocyte antigen-related deficiency enhances insulin-like growth factor-1 signaling in vascular smooth muscle cells and promotes neointima formation in response to vascular injury.||Niu XL,Li J,Hakim ZS,Rojas M,Runge MS,Madamanchi NR||The Journal of biological chemistry (282:19808)||2007|
|Mouse||Not Cited||The adapter protein GRB10 is an endogenous negative regulator of insulin-like growth factor signaling.||Dufresne AM,Smith RJ||Endocrinology (146:4399)||2005|
|Hamster||Not Cited||Interdependent regulation of insulin receptor kinase activity by ADP and hydrogen peroxide.||Schmitt TL,Hotz-Wagenblatt A,Klein H,Dröge W||The Journal of biological chemistry (280:3795)||2005|
||The adapter protein GRB10 is an endogenous negative regulator of insulin-like growth factor signaling.||Dufresne AM,Smith RJ||Endocrinology (146:4399)||2005|
||Interdependent regulation of insulin receptor kinase activity by ADP and hydrogen peroxide.||Schmitt TL,Hotz-Wagenblatt A,Klein H,Dröge W||The Journal of biological chemistry (280:3795)||2005|
CD220; CD221; HHF5; IGF-I Receptor; IGF1 Receptor; IGF1R; IGFIR; IGFR; INSR; Insulin receptor; Insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; IR; JTK13; soluble IGF1R variant 1; soluble IGF1R variant 2
CD220; CD221; HHF5; IGF1R; IGFIR; IGFR; INSR; JTK13